DNA Extraction and Purification
Zymo Research Corporation ZymoBIOMICS-96 DNA Kit (lysis Matrix Not Included) (2 x 96 preps)
The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies1 of different organisms (e.g. Gram-negative/positive bacteria including endospores2 , fungi, protozoans, algae, etc.) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard.In addition, the ZymoBIOMICS™ 96 DNA Kit is equipped with Zymo Researchs proprietary OneStep™ PCR InhibitorRemoval technology, enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ Technology results in superior yields of ultra-pure DNA ideal for all downstreamapplications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.
LGC GENOMICS LLC (Lucigen) QuickExtract Plant DNA Extraction SolutionRapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples.
QuickExtract Plant DNA Extraction SolutionRapid and efficient extraction of PCR-ready genomic DNA from plant leaf samplesFast: PCR-ready DNA in about 8 minutes for most samplesSimple: No bead-beating, freezing, or grinding of plant leaf materialIncreased Yields: No centrifugation or spin columns used which reduce yieldsCompatible: Extracted DNA is compatible with both real-time and end-point PCRSafe: Non-toxic reagents used throughout the procedure
![Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes (0.1mm & 0.5 mm) 50 pack x 4[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4308ZYMOBIOMICS96MAGBEADDNAKI.PNG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes (0.1mm & 0.5 mm) 50 pack x 4[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Agilent Technologies TRANSPACK PACKAGING EXTRACT
Transpack Packaging Extract, Ideal for recovery of all lambda shuttle vectors, No artifacts from background B-gal, Highest efficiency packaging extract available, Deficient in McrA, McrBC, Mrr and McrF restriction systems
Zymo Research Corporation Genomic DNA Wash 1, 25 ml
Genomic DNA Wash 1 (25 ml). Sold separately or as part of a nucleic acid purification kit from Zymo Research.
Sigma Aldrich Fine Chemicals Biosciences DNase I Amplification Grad
Deoxyribonuclease I (DNase I) is an endonucleaseisolatedfrom bovine pancreas that digests double and single stranded DNA into oligo and mononucleotides. Amplification Grade DNase I has been purified to remove RNase activity and is suitable for eliminating DNA from RNA preparations prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction). DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop SOLUTIONon. Heating also denatures hairpins in the RNA so the RNA can be used directly in reverse transcription.No current RNA isolation procedure removes 100% of the DNA. Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laborator
Zymo Research Corporation DNA/RNA Shield Lysis Tubes (0.1 & 0.5 mm) (Microbe) 50 pack
DNA/RNA Shield Lysis and Collection tubes are designed for the streamlined collection, transport, and processing of biological specimen. They are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization.
Zymo Research Corporation ZymoBIOMICS DNA Microprep Kit (lysis Matrix Not Included) (50 preps)
The ZymoBIOMICS DNA Microprep Kitis designed for purifying DNA from a wide array of sample inputs (e.g.feces, soil, water, and biofilms), that is immediately ready for microbiome or metagenome analyses.
Zymo Research Corporation Quick-DNA™ Viral 96 Kit (4 x 96 Preps)
Quick recovery of viral DNA from a wide range of sources using Zymo-Spin plate technology. Plate design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Zymo Research Corporation Zymoprep™ -96 Yeast Plasmid Miniprep (8 x 96 preps)
The Zymoprep™-96 Yeast Plasmid Miniprep kit provides all the necessary reagents for high-throughput plasmid isolation from S. cerevisiae, C. albicans, S. pombe and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The Zymoprep™-96 is a simple and efficient yeast plasmid miniprep kit that is based on the classic E. coli alkaline lysis method with our Zymolyase™ added in the first solution. There is no need for glass beads, or phenol.
Zymo Research Corporation ZymoBIOMICS DNA Mini Kit (5 preps)
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
New England Biolabs, Inc. NEBNext® Quick Ligation™ Module – 20 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow. This module is also compatible with some Oxford Nanopore Technologies workflows.
- Compatible with some Oxford Nanopore Technologies workflows
Zymo Research Corporation DNA Binding Buffer, 25ml
This buffer facilities efficient binding and recovery of DNA from enzymatic reactions and impure DNA samples.
Zymo Research Corporation ZR Plasmid Miniprep™-Classic Kit (100 Preps)
The ZR Plasmid Miniprep-Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. It features a modified alkaline lysis protocol together with Zymo-Spin technology to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy visualization of complete cell lysis and neutralization. The innovative Zymo-Spin IIN columns yield transfection-grade plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.
Zymo Research Corporation Quick-DNA™ MiniPrep (50 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
For easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.