DNA Extraction and Purification
Macherey-Nagel NucleoSpin Tissue XS, 250 preps
NucleoSpin Tissue XS (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue XS Columns, Collection Tubes, buffers, Proteinase K
New England Biolabs, Inc. Monarch® Spin gDNA Extraction Kit - 150 preps
The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms
New England Biolabs, Inc. Monarch® Spin PCR & DNA Cleanup Kit - 250 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 ug), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 ul, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps
Macherey-Nagel NucleoSpin Blood XL, 50 preps
NucleoSpin Blood XL (50) 50 preps for the isolation of genomic DNA from blood - NucleoSpin Blood XL Columns, Collection Tubes, buffers, Proteinase K
Takara Bio NucleoBond® Xtra Midi Plus, 50 preps
NucleoBond Xtra Midi Plus (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, NucleoBond Finalizers, syringes, buffers, RNase A.
SignaGen Laboratories GENJET DNA REAGENT HELA 1ML
GenJet DNA In Vitro Tranfection Reagent for Hela was pre-optimized for most efficiently transfecting Hela cells.
Quantitative Genomic RNA from Influenza A virus H1N1 strain A/California/07/2009 (H1N1)pdm09 (ATCC VR-1894DQ™)
Quantitative Genomic RNA from Influenza A virus H1N1 strain A/California/07/2009 (H1N1)pdm09 (ATCC VR-1894DQ™) is a molecular standard used for qPCR assay development, validation, and viral load quantification. This product is recommended for respiratory disease research and vaccine-related assay workflows.
ATCC Quantitative Genomic; RNA from Influenza; B virus; Strain: B/Lee/40
Quantitative Genomic RNA from Influenza B virus strain B/Lee/40 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Macherey-Nagel NucleoSpin Gel and PCR Clean-up Midi, 20 preps
NucleoSpin Gel and PCR Clean-up Midi(20) 20 preps for large scale PCR clean-up and gel extraction, Buffers, NucleoSpin Extract II Midi Columns, Collection Tubes, User Manual
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
New England Biolabs, Inc. Monarch® HMW DNA Extraction Kits for Cells & Blood - 5 preps
The Monarch HMW DNA Extraction Kit for Cells & Blood provides a rapid and reliable process for extracting high molecular weight (HMW), intact genomic DNA from cultured cells and whole blood. Utilizing an optimized process that combines gentle cell lysis with a tunable fragment length generation followed by precipitation of the extracted DNA onto the surface of large glass beads, the prep proceeds rapidly and utilizes standard laboratory equipment. DNA size ranges from 50-250 kb for the standard protocol and into the Mb range when the lowest agitation speeds are used. Purified DNA is recovered in high yield with excellent purity, including nearly complete removal of RNA. For cells, the process time is only 30 min, while blood samples require erythrocyte lysis and are processed in 60 min